eclipse ti-2e inverted microscope  (Nikon)

Journal: Journal of Nanobiotechnology

Article Title: A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

doi: 10.1186/s12951-022-01515-5

Figure Lengend Snippet: a Chemical structures and their corresponding fluorescence regions of organic dyes, including OPVs [242 (NIR)], DSSCs [YI-1 (NIR), YI-3 (red), YI-8 (red)], OLEDs [ADF1 (red), ADF-2 (green), ADF-3 (blue), and DTDPTID (NIR)], and commercial materials [Rhodamine 6G (R6G) (orange-red), sodium iron chlorophyllin (FeChl) (red), and CY5 (red)]. b Schematic illustration for the sponge-like swelling nature of the PSMA NPs capable of physical adsorption these photosensitive molecules into the interspace. c The utility of the CW and fs laser to make the dye-loaded PSMA NPs fluorescent induction (via single-photon and two-photon absorption) of single-photon/multiphoton imaging in cellular and blood vessel and NIR-PDT (via an intersystem crossing pathway) in bladder tumor

Article Snippet: MDA-MB-231 cells (2 × 10 5 /mL, 1 mL) were seeded in confocal dishes and cultured with Dulbecco’s modified Eagle’s medium with 1% (v/v) penicillin/streptomycin and 10% (v/v) FBS for 24 h. Afterward, nanoparticles (PSMA-242, 2.4 ppm, 1 mL; PSMA-YI-1, 11.8 ppm, 1 mL) were added to the dishes, and the cells were cultured for another 16 h. Then, the cells were washed with medium three times and observed using a Nikon Inverted Multiphoton Microscope Eclipse (A1MP + Eclipse Ti-2E, Nikon instrument Inc., Japan) with a 40 × NA = 1.15 water-immersion objective.

Techniques: Fluorescence, Adsorption, Imaging

Journal: Journal of Nanobiotechnology

Article Title: A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

doi: 10.1186/s12951-022-01515-5

Figure Lengend Snippet: Absorption and multiphoton fluorescence spectra of PSMA loaded with dyes with high molar masses. a UV–Vis spectra and b single-photon fluorescent spectra (excited at 512 nm) of 242, YI-1 (solid line), PSMA-242, and PSMA-YI-1 NPs (dotted line). c Fluorescence spectra of ADF1 and PSMA-ADF1 NPs excited at 512 nm. Multiphoton fluorescence spectra of d PSMA-242, e PSMA-YI-1, and f PSMA-ADF1 NPs

Article Snippet: MDA-MB-231 cells (2 × 10 5 /mL, 1 mL) were seeded in confocal dishes and cultured with Dulbecco’s modified Eagle’s medium with 1% (v/v) penicillin/streptomycin and 10% (v/v) FBS for 24 h. Afterward, nanoparticles (PSMA-242, 2.4 ppm, 1 mL; PSMA-YI-1, 11.8 ppm, 1 mL) were added to the dishes, and the cells were cultured for another 16 h. Then, the cells were washed with medium three times and observed using a Nikon Inverted Multiphoton Microscope Eclipse (A1MP + Eclipse Ti-2E, Nikon instrument Inc., Japan) with a 40 × NA = 1.15 water-immersion objective.

Techniques: Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

doi: 10.1186/s12951-022-01515-5

Figure Lengend Snippet: a Biocompatibility of PSMA NPs loaded with different dyes. Fluorescent images of cells labeled with b PSMA-242, YI-1, ADF1 single staining or c PSMA-(ADF1 + ADF2) double staining and tracked by fluorescence microscopy. Scale bars = 20 μm. Multiphoton imaging of d PSMA-242 and e PSMA-YI-1 excited at 1080 nm. In vivo two-photon fluorescence (red color) and second harmonic generation (green color) imaging of dye-loaded PSMA NPs circulated in the blood vessels of a mouse ear. f PSMA-242; g PSMA-YI-1; excitation wavelength: 1080 nm. Fields of view: 317 × 317 μm

Article Snippet: MDA-MB-231 cells (2 × 10 5 /mL, 1 mL) were seeded in confocal dishes and cultured with Dulbecco’s modified Eagle’s medium with 1% (v/v) penicillin/streptomycin and 10% (v/v) FBS for 24 h. Afterward, nanoparticles (PSMA-242, 2.4 ppm, 1 mL; PSMA-YI-1, 11.8 ppm, 1 mL) were added to the dishes, and the cells were cultured for another 16 h. Then, the cells were washed with medium three times and observed using a Nikon Inverted Multiphoton Microscope Eclipse (A1MP + Eclipse Ti-2E, Nikon instrument Inc., Japan) with a 40 × NA = 1.15 water-immersion objective.

Techniques: Labeling, Staining, Double Staining, Fluorescence, Microscopy, Imaging, In Vivo